Journal: The Kaohsiung Journal of Medical Sciences

Article Title: LRP8 Regulates Lipid Metabolism to Stimulate Malignant Progression and Cisplatin Resistance in Bladder Cancer

doi: 10.1002/kjm2.70042

Figure Lengend Snippet: LRP8 is upregulated in BCa and is related to a dismal prognosis. (A) TCGA database analysis of the LRP8 expression in tumor tissues and adjacent tissues. (B) IHC detection of the LRP8 expression in tumor tissues and adjacent tissues ( n = 10). The left picture is the representative image. (C) QRT‐PCR detection of the LRP8 expression in immortalized human uroepithelial cells and BCa cells. (D) The Kaplan–Meier survival curve was used to analyze the overall survival of patients with high or low LRP8 expression. * indicates p < 0.05, which means a significant difference. All experiments were repeated three times. BCa, bladder cancer; IHC, Immunohistochemistry; LRP8, low‐density lipoprotein receptor‐related protein 8; qRT‐PCR, Quantitative reverse transcription polymerase chain reaction; TCGA, The Cancer Genome Atlas Program.

Article Snippet: BCa cells (T24, RT4, 5637) and the immortalized human uroepithelial cell line (SV‐HUC‐1) were acquired from Procell Life Science & Technology (China).

Techniques: Expressing, Quantitative RT-PCR, Immunohistochemistry, Reverse Transcription, Polymerase Chain Reaction

Journal: The Journal of International Medical Research

Article Title: Pachymic acid alleviates lipopolysaccharide-induced cystitis glandularis by inhibiting ferroptosis

doi: 10.1177/03000605251396755

Figure Lengend Snippet: Therapeutic effect of PA on LPS-induced CG. (a) Histopathological sections of bladder tissues from each group were observed via H&E staining. The magnified regions revealed the epithelial layer of the bladder. In the LPS group, distinct formation of Brunn’s nests was observed, accompanied by epithelial edema and increased inflammatory cell infiltration. Following treatment with Fer-1 and PA, Brunn’s nests disappeared, epithelial edema was alleviated, and inflammatory cell infiltration was reduced (Scale bar = 100 μm). (b) At 400× magnification, histopathological features across multiple fields of view were observed. The severity of inflammation was scored and visualized as a heatmap, where columns represent three individual animals per group, and rows correspond to five distinct microscopic fields from each animal. Additionally, statistical analysis of the inflammatory scores was performed. (c) to (e) Levels of IL-1β, IL-6, and TNF-α in bladder tissues from each group of rats. The above data are presented as mean value ± SD, ns means p > 0.05, *p < 0.05, **p < 0.01, ***p < 0.001. CG: cystitis glandularis; H&E: hematoxylin and eosin; LPS: lipopolysaccharide; PA: pachymic acid.

Article Snippet: Human bladder epithelial cells (SV40-immortalized human urothelial cells-1 (SV-HUC-1)) and Sprague–Dawley rat bladders were treated with lipopolysaccharide, with pachymic acid and ferrostatin-1 used as interventions.

Techniques: Staining

Journal: Open Medicine

Article Title: ARAF regulates malignant progression of bladder cancer through the p38MAPK pathway

doi: 10.1515/med-2026-1422

Figure Lengend Snippet: Expression levels of ARAF in bladder cancer cells. (A) mRNA expression of ARAF in different cell lines. (B and C) Protein expression of ARAF in different cell lines. Data are presented as mean ± SD from three independent biological replicates. Statistical analysis was performed using one-way ANOVA. ***p<0.001, ****p<0.0001 vs. SV-HUC-1.

Article Snippet: The human ureter epithelial immortalized cell line SV-HUC-1 (CL-0222) and human bladder cancer cell lines T24 (CL-0227) and J82 (CL-0125) were purchased from Wuhan Procell Biological Company.

Techniques: Expressing